Pin 1 Functional PPIase Assay

  • Pin1 is a PPIase specific for pSer/pThr-Pro motifs
  • Uses recombinant human Pin 1 and substrate Succ-AEPF-pNA
  • Measures cis/trans isomerisation kinetics of substrate using UV/Vis spectrophotometer
  • Uses LiCl / Trifluoroethanol. Li+ ion holds substrate in 60% cis conformation
  • Ki values obtained (6pt in duplicates)
  • Can be performed with and without additives (detergent/salt)

Click here to see how the functional PPIase assay works

*Reference:- Wildemann et al; J Med. Chem 2006, 49, 2147